Thin Layer Chromatography

Posted on Mar 05, 2016 by Rajesh

Thin layer chromatography (TLC) is used for identifying the compounds, finding out their purity and optimizing the solvent by separating the desired compound. To carry out the separation using this TLC technique, an adsorbent in the powdered form is used as a thin layer. Here, stationary phase consists of solid adsorbent such as silica or alumina coated on glass, plastic support, whereas the mobile phase consists of a solvent or mixture of the solvents. This material is then applied on the glass plate, aluminum foil or plastic sheet or plate.

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