This process is highly efficient, durable and offers quick result in order to optimizing the resolution. It is also useful in extracting and purifying the range of sample component. It has the ability to speed up the process and improve the performance of the separation by enhancing the mobile phase actions to pass through the columns with great efficient. Many chromatographic separation processes need to use certain analytical tools that help in carrying out the procedure effectively and precisely. The wide range of detection and analysis methods are used to handle number of compounds at the same time.